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Proteintech cyclin d1
Cyclin D1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclin+d1/Cyclin+D1+Antibody/pm42929872-128-147-150
Average 96 stars, based on 1630 article reviews
cyclin d1 - by Bioz Stars, 2026-11
96/100 stars

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Related Articles

Knock-Out:

Article Title: CGP60474, a novel MITD1-targeted inhibitor for bladder cancer progression via CDK4/cyclin D1 pathway modulation
Article Snippet: .. Antibodies against MITD1 (17264-1-AP, Proteintech), Tubulin (66240-1-Ig, Proteintech), β-Actin (66009-1-Ig, Proteintech), CDK4 (11026-1-AP, 66950-1-Ig, Proteintech), cyclin D1 (26939-1-AP, 60186-1-Ig, Proteintech), DYKDDDDK tag Monoclonal Fig. 7 | Genetic knockout ofMITD1 via CRISPR-Cas9 impairs proliferation and induces cell cycle arrest in UMUC-3 cells. ..

CRISPR:

Article Title: CGP60474, a novel MITD1-targeted inhibitor for bladder cancer progression via CDK4/cyclin D1 pathway modulation
Article Snippet: .. Antibodies against MITD1 (17264-1-AP, Proteintech), Tubulin (66240-1-Ig, Proteintech), β-Actin (66009-1-Ig, Proteintech), CDK4 (11026-1-AP, 66950-1-Ig, Proteintech), cyclin D1 (26939-1-AP, 60186-1-Ig, Proteintech), DYKDDDDK tag Monoclonal Fig. 7 | Genetic knockout ofMITD1 via CRISPR-Cas9 impairs proliferation and induces cell cycle arrest in UMUC-3 cells. ..

Incubation:

Article Title: Sphingolipid metabolic subtyping defines SPHK1 as an oncogene and characterizes immunosuppressive TME features in gastric cancer
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE and transferred onto PVDF membranes (Millipore, United States). .. Membranes were blocked with 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies against SPHK1 (1:1000; Abcam, ab71700), Bax (1:1000; Cell Signaling Technology, #2772), CDC2 (1:1000; Abcam, ab18), Cyclin D1 (1:2000; Proteintech, 26939-1-AP), p21 (1:1000; Cell Signaling Technology, #2947), p27 (1:1000; Cell Signaling Technology, #3686), TGF-β1 (1:500; R&D Systems, AF-246-NA), N-cadherin (1:5000; Sigma-Aldrich, C3865), E-cadherin (1:5000; Sigma-Aldrich, MAB3199), and α-tubulin (1:5000; Sigma-Aldrich, T6199). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Cell Signaling Technology) for 1 h at room temperature.

Article Title: A comprehensive analysis of common cytogenetic abnormalities in multiple myeloma: from basic research to clinical applications.
Article Snippet: The significant heterogeneity of multiple myeloma (MM) profoundly impacts patient prognosis, with cytogenetic abnormalities serving as a core driver.. Based on the International Myeloma Working Group and 2024 International Myeloma Society consensus, this study enrolled 52 newly diagnosed MM patients.. Six cytogenetic abnormalities—RB1 deletion, D13S319 deletion, immunoglobulin heavy chain (IGH) translocation, 1q21 gain/ amplification, P53 deletion, and 1p32 deletion—were detected using fluorescence in situ hybridisation.

Article Title: The pro-obesity role of G3BP1 through altering adipogenic differentiation in mice via the PI3K/AKT pathway.
Article Snippet: After being separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on gels ranging from 7 to 12%, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Billerica, MA, USA). .. After being blocked with 5% skim milk at room temperature for 1 h, the membranes were incubated with the primary antibodies against G3BP1 (66486–1-Ig, 1/5000, Proteintech, Wuhan, China), proliferating cell nuclear antigen (PCNA; 10,205–2-AP, 1/5000, Proteintech, Wuhan, China), cyclin‐dependent kinase 2 (CDK2; 10,122–1-AP, 1/5000, Proteintech, Wuhan, China), cyclin B1 (CCNB1; 55,004–1-AP, 1/1000, Proteintech, Wuhan, China), cyclin D2 (CCND2; 10,934–1-AP, 1/1000, Proteintech, Wuhan, China), matrix metalloprotease 9 (MMP9; 82,854–8-RR, 1/5000, Proteintech, Wuhan, China), matrix metalloprotease 7 (MMP7; 10,374–2-AP, 1/1000, Proteintech, Wuhan, China), matrix metalloprotease 2 (MMP2; 10,373–2-AP, 1/1000, Proteintech, Wuhan, China), peroxisome proliferator-activated receptor gamma (PPARγ; 16,643–1-AP, 1/1000, Proteintech, Wuhan, China), CCAAT/enhancer‐binding protein alpha (CEBPα; 29,388–1-AP, 1/1000, Proteintech, Wuhan, China), adiponectin receptor 1 (ADIPOR1; 66,619–1-Ig, 1/1000, Proteintech, Wuhan, China), fatty acid‐binding protein 4 (FABP4; 12,802–1-AP, 1/5000, Proteintech, Wuhan, China), leptin receptor (LEPR; 20,966–1- AP, 1/5000, Proteintech, Wuhan, China), fatty acid synthase (FAS; 13,098–1-AP, 1/5000, Proteintech, Wuhan, China), adipose triglyceride lipase (ATGL; 55,190–1-AP, 1/5000, Proteintech, Wuhan, China), lipoprotein lipase (LPL; 28,602–1-AP, 1/1000, Proteintech, Wuhan, China), fat mass and obesityassociated protein (FTO; 27,226–1-AP, 1/1000, Proteintech, Wuhan, China), β-catenin (51067–2-AP, 1/5000, Proteintech, Wuhan, China), cyclin D1 (26939–1-AP, 1/5000, Proteintech, Wuhan, China), cMyc (67447–1-Ig, 1/5000, Proteintech, Wuhan, China), axis inhibition protein 2 (Axin2; 20,540–1-AP, 1/1000, Proteintech, Wuhan, China), p-PI3K (bs-5570 R, 1/1000, Bioss, Beijing, China), PI3K (20584–1- AP, 1/1000, Proteintech, Wuhan, China), p-AKT (66444–1-Ig, 1/2000, Proteintech, Wuhan, China), AKT (10176–2-AP, 1/2000, Proteintech, Wuhan, China), resistin (18170–1-AP, 1/2000, Proteintech, Wuhan, China), sterol regulatory element-binding protein 1 (SREBP-1; 66,875–1-Ig, 1/2000, Proteintech, Wuhan, China), acyl-CoA synthetase long-chain family member 1 (ACSL1; FNab00106, 1/1000, FineTest, Wuhan, China), and GAPDH (60004–1-Ig, 1/50000, Proteintech, Wuhan, China) at 4°C overnight and with horseradish peroxidase (HRP)-conjugated secondary antibodies (SA00001-2, 1/5000; Proteintech, Wuhan, China) for 1 h at room temperature. .. The protein bands were visualized using enhanced chemiluminescence (ECL) ultrasensitive luminescence reagent (Thermofisher, MA, USA) on a Davinch-Chemi Imager.

Cell-Signaling:

Article Title: Sphingolipid metabolic subtyping defines SPHK1 as an oncogene and characterizes immunosuppressive TME features in gastric cancer
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE and transferred onto PVDF membranes (Millipore, United States). .. Membranes were blocked with 5% non-fat milk in TBST and incubated overnight at 4 °C with primary antibodies against SPHK1 (1:1000; Abcam, ab71700), Bax (1:1000; Cell Signaling Technology, #2772), CDC2 (1:1000; Abcam, ab18), Cyclin D1 (1:2000; Proteintech, 26939-1-AP), p21 (1:1000; Cell Signaling Technology, #2947), p27 (1:1000; Cell Signaling Technology, #3686), TGF-β1 (1:500; R&D Systems, AF-246-NA), N-cadherin (1:5000; Sigma-Aldrich, C3865), E-cadherin (1:5000; Sigma-Aldrich, MAB3199), and α-tubulin (1:5000; Sigma-Aldrich, T6199). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Cell Signaling Technology) for 1 h at room temperature.

other:

Article Title: Zhuche Pill alleviates DSS-induced colonic epithelial injury in mice: involvement of IL-17/NF-κB and Wnt/β-catenin signaling pathways.
Article Snippet: Ethnopharmacological relevance: Zhuche Pill (ZCP), a traditional Chinese medicine formula first documented in Qianjin Yaofang during the Tang Dynasty, has been used for centuries to treat dysentery-associated gastrointestinal disorders, including abdominal pain, diarrhea, hematochezia, and tenesmus.. Despite the long-standing clinical application of ZCP, the pharmacological basis underlying its therapeutic effects remains incompletely understood.. Aim of the study: This study aims to evaluate the protective effects of ZCP against colitis and explore its potential underlying mechanisms.

Article Title: Tirzepatide Attenuates Pulmonary Vascular Remodeling in Pulmonary Arterial Hypertension: Association with the SIRT1/H4K12la/c-Jun Axis.
Article Snippet: The membranes were incubated in 5% non-fat milk diluted in TBST for 1 h at room temperature, and the blots were then probed overnight at 4 ◦C with specific primary antibodies [GLP1R (26196, Proteintech, Wuhan, China), GIPR (87123, Proteintech, Wuhan, China), Col1 (72026, Cell Signaling Technology, Danvers, MA, USA), MMP9 (13667, Cell Signaling Technology, Danvers, MA, USA), CNN1 (17819, Cell Signaling Technology, Danvers, MA, USA), α-SMA (14359, Proteintech, Wuhan, China), β-actin (66009, Proteintech, Wuhan, China), GAPDH (60004, Proteintech, Wuhan, China), L-Lactyl-Histone Antibody Sampler Kit (PTM-7093, PTM Bio, Hangzhou, China), HDAC1 (10197, Proteintech, Wuhan, China), HDAC2 (12922, Proteintech, Wuhan, China), HDAC3 (10255, Proteintech, Wuhan, China), SIRT1 (13161, Proteintech, Wuhan, China), SIRT3 (10099, Proteintech, Wuhan, China), p-AMPK (2531, Cell Signaling Technology, Danvers, MA, USA), AMPK (2795, Cell Signaling Technology, Danvers, MA, USA), p-JUN (3270, Cell Signaling Technology, Danvers, MA, USA), c-JUN (9165, Cell Signaling Technology, Danvers, MA, USA), and Cyclin D1 (60186, Proteintech, Wuhan, China)].

Inhibition:

Article Title: The pro-obesity role of G3BP1 through altering adipogenic differentiation in mice via the PI3K/AKT pathway.
Article Snippet: After being separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on gels ranging from 7 to 12%, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Billerica, MA, USA). .. After being blocked with 5% skim milk at room temperature for 1 h, the membranes were incubated with the primary antibodies against G3BP1 (66486–1-Ig, 1/5000, Proteintech, Wuhan, China), proliferating cell nuclear antigen (PCNA; 10,205–2-AP, 1/5000, Proteintech, Wuhan, China), cyclin‐dependent kinase 2 (CDK2; 10,122–1-AP, 1/5000, Proteintech, Wuhan, China), cyclin B1 (CCNB1; 55,004–1-AP, 1/1000, Proteintech, Wuhan, China), cyclin D2 (CCND2; 10,934–1-AP, 1/1000, Proteintech, Wuhan, China), matrix metalloprotease 9 (MMP9; 82,854–8-RR, 1/5000, Proteintech, Wuhan, China), matrix metalloprotease 7 (MMP7; 10,374–2-AP, 1/1000, Proteintech, Wuhan, China), matrix metalloprotease 2 (MMP2; 10,373–2-AP, 1/1000, Proteintech, Wuhan, China), peroxisome proliferator-activated receptor gamma (PPARγ; 16,643–1-AP, 1/1000, Proteintech, Wuhan, China), CCAAT/enhancer‐binding protein alpha (CEBPα; 29,388–1-AP, 1/1000, Proteintech, Wuhan, China), adiponectin receptor 1 (ADIPOR1; 66,619–1-Ig, 1/1000, Proteintech, Wuhan, China), fatty acid‐binding protein 4 (FABP4; 12,802–1-AP, 1/5000, Proteintech, Wuhan, China), leptin receptor (LEPR; 20,966–1- AP, 1/5000, Proteintech, Wuhan, China), fatty acid synthase (FAS; 13,098–1-AP, 1/5000, Proteintech, Wuhan, China), adipose triglyceride lipase (ATGL; 55,190–1-AP, 1/5000, Proteintech, Wuhan, China), lipoprotein lipase (LPL; 28,602–1-AP, 1/1000, Proteintech, Wuhan, China), fat mass and obesityassociated protein (FTO; 27,226–1-AP, 1/1000, Proteintech, Wuhan, China), β-catenin (51067–2-AP, 1/5000, Proteintech, Wuhan, China), cyclin D1 (26939–1-AP, 1/5000, Proteintech, Wuhan, China), cMyc (67447–1-Ig, 1/5000, Proteintech, Wuhan, China), axis inhibition protein 2 (Axin2; 20,540–1-AP, 1/1000, Proteintech, Wuhan, China), p-PI3K (bs-5570 R, 1/1000, Bioss, Beijing, China), PI3K (20584–1- AP, 1/1000, Proteintech, Wuhan, China), p-AKT (66444–1-Ig, 1/2000, Proteintech, Wuhan, China), AKT (10176–2-AP, 1/2000, Proteintech, Wuhan, China), resistin (18170–1-AP, 1/2000, Proteintech, Wuhan, China), sterol regulatory element-binding protein 1 (SREBP-1; 66,875–1-Ig, 1/2000, Proteintech, Wuhan, China), acyl-CoA synthetase long-chain family member 1 (ACSL1; FNab00106, 1/1000, FineTest, Wuhan, China), and GAPDH (60004–1-Ig, 1/50000, Proteintech, Wuhan, China) at 4°C overnight and with horseradish peroxidase (HRP)-conjugated secondary antibodies (SA00001-2, 1/5000; Proteintech, Wuhan, China) for 1 h at room temperature. .. The protein bands were visualized using enhanced chemiluminescence (ECL) ultrasensitive luminescence reagent (Thermofisher, MA, USA) on a Davinch-Chemi Imager.



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